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半胱氨酸残基的溶剂可及性分析<em>玉米rayado菲诺病毒</em病毒样颗粒<em>烟草</em植物和交联的肽类病毒颗粒
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs
DOI:

08:14 min

February 14, 2013

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Chapters

  • 00:05Title
  • 01:30Virus Inoculation of Nicotiana benthamiana and Fluorescein-5-maleimide-labeling of Virus-Like Particles
  • 03:33Cross-linking of Cys 2-VLPs and F Peptide
  • 04:48Representative VLP-Peptide Crosslinking Results
  • 08:01Conclusion

Summary

Automatic Translation

的方法来分析半胱氨酸残基的巯基的溶剂可及<em>玉米rayado菲诺病毒</em>(MRFV) - 病毒样颗粒(VLPs)随后的肽交联反应的说明。的方法,利用几个化学基团的表面上的,可以是特定的反应,目标的病毒样颗粒的可用性。

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