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Neonatale subventriculaire zone Elektroporatie
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Neonatal Subventricular Zone Electroporation
DOI:

08:06 min

February 11, 2013

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Chapters

  • 00:05Title
  • 01:39Preparation of DNA, Solutions, and Glass Pipettes
  • 02:54Animal Preparation, Glass Pipette Loading and Plasmid Injection
  • 05:04Electroporation and Recovery
  • 06:44Results: Images of an Electroporated Subventricular Zone
  • 07:48Conclusion

Summary

Automatic Translation

We tonen een minimaal invasieve techniek genoemd neonatale subventriculaire zone elektroporatie. De techniek bestaat uit het injecteren plasmide DNA in de laterale ventrikels van neonatale pups en toepassen elektrische stroom te leveren en genetisch manipuleren neurale stamcellen

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