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Neuroscience
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线粒体相关的ER膜(MAMS)和鞘糖脂丰富的微区(GEMS):分离小鼠脑
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Neuroscience
Mitochondria-associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs): Isolation from Mouse Brain
Please note that all translations are automatically generated.
Click here for the English version.
线粒体相关的ER膜(MAMS)和鞘糖脂丰富的微区(GEMS):分离小鼠脑
DOI:
10.3791/50215-v
•
10:32 min
•
March 04, 2013
•
Ida Annunziata*
1
,
Annette Patterson*
1
,
Alessandra d’Azzo
1
Department of Genetics
,
St Jude Children’s Research Hospital
Chapters
00:05
Title
02:21
Subcellualar Fractionation: Removal of Nuclei, Unlysed Cells, and Cellular Debris
04:08
Crude Mitochondria Isolation
06:16
Isolation of Mitochondria-associated ER Membrane, MAMs
08:27
Extraction of Glycosphingolipid-enriched Microdomains, GEMs
09:14
Results: Western Blot Analysis of GEMs and MAMs
10:01
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
此过程说明了如何分离从成年小鼠的大脑相关的线粒体的ER膜或MAMS和糖脂富集的微区部分从MAMS和线粒体的准备工作。
Tags
Mitochondria-associated ER Membranes (MAMs)
Glycosphingolipid Enriched Microdomains (GEMs)
Intracellular Organelles
Endoplasmic Reticulum (ER)
Mitochondria
Ca2+ Signaling
Lipid Biosynthesis
Energy Metabolism
Cell Survival
Membrane Contact Sites
Triton X100 Extraction
Caveolae
Lipid Rafts
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