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线粒体相关的ER膜（MAMS）和鞘糖脂丰富的微区（GEMS）：分离小鼠脑

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Mitochondria-associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs): Isolation from Mouse Brain

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线粒体相关的ER膜（MAMS）和鞘糖脂丰富的微区（GEMS）：分离小鼠脑

DOI:

10.3791/50215-v

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10:32 min

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March 04, 2013

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Ida Annunziata*¹, Annette Patterson*¹, Alessandra d’Azzo

¹Department of Genetics,St Jude Children’s Research Hospital

Chapters

00:05Title
02:21Subcellualar Fractionation: Removal of Nuclei, Unlysed Cells, and Cellular Debris
04:08Crude Mitochondria Isolation
06:16Isolation of Mitochondria-associated ER Membrane, MAMs
08:27Extraction of Glycosphingolipid-enriched Microdomains, GEMs
09:14Results: Western Blot Analysis of GEMs and MAMs
10:01Conclusion

Summary

Automatic Translation

此过程说明了如何分离从成年小鼠的大脑相关的线粒体的ER膜或MAMS和糖脂富集的微区部分从MAMS和线粒体的准备工作。

Tags

Mitochondria-associated ER Membranes (MAMs)Glycosphingolipid Enriched Microdomains (GEMs)Intracellular Organelles Endoplasmic Reticulum (ER)Mitochondria Ca2+ Signaling Lipid Biosynthesis Energy Metabolism Cell Survival Membrane Contact Sites Triton X100 Extraction Caveolae Lipid Rafts

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