Journal
/
/
Развитие, расширение и<em> В естественных условиях</em> Контроль за соблюдением NK-клетки из человеческих эмбриональных стволовых клеток (ЭСК) и и индуцированных плюрипотентных стволовых клеток (ИПСК)
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)
DOI:

09:02 min

April 23, 2013

, ,

Chapters

  • 00:05Title
  • 01:18Setting up TrypLE-passaged ES Cells into Stage I Spin Embryoid Bodies (EBs): Day of Spin EB Setup into Stage I Differentiation (Day 0)
  • 03:16Development, Expansion, and Characterization of NK Cells from hPSC-derived Progenitor Cells
  • 04:19In vivo Fluorescent and Bioluminescent Imaging to Monitor hESC-derived NK Cells and Tumor Cells in Immunodeficient Mice
  • 06:50Results: The Generation of Hematopoietic Progenitor Cells Using the Spin EB Approach Allows for Optimal NK Cell Development from hESCs and iPSCs
  • 08:43Conclusion

Summary

Automatic Translation

Этот протокол описывает развитие, расширение, а в естественных изображений NK клетки, полученные из ЭСК и ИПСК.

Related Videos

Read Article