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高效，快速的早期胚胎分离<em>拟南芥</em>种子
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JoVE Journal Biology
Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds
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高效，快速的早期胚胎分离<em>拟南芥</em>种子

DOI:
10.3791/50371-v

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08:05 min

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June 07, 2013

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, , , ,
1Institute of Plant Biology and Zürich-Basel Plant Science Center,University of Zürich

Chapters

  • 00:05Title
  • 01:09Material Preparation
  • 02:24Seed Dissection and Embryo Extraction
  • 03:49Embryo Isolation
  • 06:23Results: Isolated Plant Embryos Are Suitable for Diverse Downstream Applications
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

我们报告了一种简单有效的方法分离胚胎在早期发展阶段<em>拟南芥</em>种子。多达40个胚胎可以在1小时至4小时，分离根据下游应用。该程序是适用于转录，DNA甲基化，报告基因的表达，免疫荧光<em在原位</em>杂交分析。

Tags

Arabidopsis ThalianaEarly-stage EmbryosIsolationEndospermSeed IntegumentsSeed CoatManual DissectionGlass MicrocapillaryRT-PCRRNA SequencingDNA MethylationFISHImmunostainingReporter Gene Assays

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