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成像和胚胎斑马鱼脑血管结构的三维重建
JoVE Journal
Biology
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JoVE Journal Biology
Imaging and 3D Reconstruction of Cerebrovascular Structures in Embryonic Zebrafish
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成像和胚胎斑马鱼脑血管结构的三维重建

DOI:
10.3791/50417-v

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08:00 min

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April 22, 2014

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1Molecular Neurobiology,Western University of Health Sciences, 2Graduate College of Biomedical Sciences,Western University of Health Sciences, 3College of Osteopathic Medicine of the Pacific,Western University of Health Sciences, 4College of Optometry,Western University of Health Sciences

Chapters

  • 00:05Title
  • 01:23Zebrafish Husbandry, Embryo Generation, and Treatment
  • 02:50Confocal Imaging of Cerebrovascular Structures in Fixed Zebrafish Embryos
  • 04:073D Reconstruction of Embryonic Zebrafish Cerebrovasculature
  • 06:34Results: 3D Reconstruction of Embryonic Zebrafish Cerebrovasculature
  • 07:26Conclusion

Summary

Automatic Translation

Automatic Translation

在斑马鱼幼虫脑血管病的发展成像描述。还提供技术，以促进3D成像，并使用化学处理修改脑发展。

Tags

Zebrafish3D ReconstructionCerebrovascular StructuresEmbryonicImagingConfocal MicroscopyEGFPVascular Endothelial CellsBrainRetinaDevelopmental BiologyPathologyIn Vivo

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