Journal
/
/
ラット中枢神経系および末梢リンパ節の組織切片での免疫組織化学的解析
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
DOI:

09:11 min

November 14, 2016

, , , , ,

Chapters

  • 00:05Title
  • 01:46Tissue Preparation: Fixation, Dehydration and Embedding
  • 02:50Tissue Sectioning, Mounting and Deparaffinization
  • 04:32Antigen Retrieval, Blocking, and Incubation with the Primary Antibodies
  • 06:38Visualization of the Bound Biotinylated Secondary Antibody
  • 07:48Results: Double Immunostaining in the Rat Lymph Nodes and the Central Nervous System
  • 08:42Conclusion

Summary

Automatic Translation

We here present an optimized, detailed protocol for double immunostaining in formalin-fixed, paraffin-embedded rat central nervous system (CNS) and peripheral lymph node (LN) tissue sections.

Related Videos

Read Article