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富亮氨酸的代谢标记重复激酶1和2与放射性磷酸盐
JoVE Journal
Biology
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JoVE Journal Biology
Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate
DOI:

11:31 min

September 18, 2013

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Chapters

  • 00:05Title
  • 02:02Metabolic Labeling of Cells
  • 05:50Analysis of Protein Labeling
  • 09:46Results: Metabolic Labeling of LRRK1 and LRRK2
  • 10:57Conclusion

Summary

Automatic Translation

富含亮氨酸的重复激酶1和2(LRRK1和LRRK2)是多结构域蛋白,其编码GTP酶都和激酶结构域和它们磷酸化的细胞中。在这里,我们提出了一个协议,以32磷正磷酸盐标记LRRK1和LRRK2的细胞,从而提供衡量其整体的细胞磷酸化水平的一种手段。

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