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双分子荧光互补流式细胞仪分析:高通量的定量方法研究蛋白质 - 蛋白质相互作用
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JoVE Journal
Biology
Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction
Please note that all translations are automatically generated.
Click here for the English version.
双分子荧光互补流式细胞仪分析:高通量的定量方法研究蛋白质 - 蛋白质相互作用
DOI:
10.3791/50529-v
•
11:11 min
•
August 15, 2013
•
Li Wang
,
Graeme K. Carnegie
1
Department of Pharmacology
,
University of Illinois at Chicago
Chapters
00:05
Title
02:05
Cell Transfection
03:46
Cell Preparation for Flow Cytometry, Western Blot, and Immunofluorescence
05:12
Flow Cytometry and Immunofluorescence Microscopy
06:50
Data Analysis
07:49
Assessing AKAP-Lbc and PDE4D3 Interaction Using BiFC-Flow Cytometry
10:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
流式细胞仪分析双分子荧光互补提供了一种高通量定量研究蛋白质相互作用的方法。此方法可用于测绘的蛋白结合位点,并用于筛选调节蛋白 - 蛋白相互作用的因素。
Tags
Flow Cytometry
Bimolecular Fluorescence Complementation
BiFC
Protein-protein Interaction
AKAP-Lbc
PDE4D3
Fluorescent Protein
High Throughput
Quantification
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