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Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry
DOI:

14:09 min

December 12, 2013

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Chapters

  • 00:05Title
  • 01:38Preparation of Water Dispersible Carbon Nanotubes
  • 03:24ImageStream Acquisition
  • 04:07Post Processing: IDEAS Analysis Software (Version 4.0)
  • 08:52Results: Representative Analyses of CNT Internalization
  • 13:37Conclusion

Summary

Automatic Translation

In this study, the main purpose was to monitor the cellular uptake and eventual exocytosis of carbon nanotubes (CNTs). From this perspective, we proposed here a unique method using multispectral imaging flow cytometry allowing a quantification and localization of CNTs in a statistically relevant number of cells.

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