Journal
/
/
氧微环境中的单个胰岛水平的定量和临时控制
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Quantitative and Temporal Control of Oxygen Microenvironment at the Single Islet Level
DOI:

11:49 min

November 17, 2013

, , , , , ,

Chapters

  • 00:05Title
  • 01:59Microfluidic Platform Fabrication
  • 05:17Microdispenser Setup
  • 06:53Isolation and Staining of Mouse Islets
  • 08:25Simultaneous Oxygen and Glucose Stimulations
  • 09:19Results: Intermittent Hypoxia Preconditioning Improves Islet Responses to Hypoxia
  • 10:46Conclusion

Summary

Automatic Translation

微流控氧赋予的不仅仅是方便和速度超过低氧室的生物实验。通过扩散穿过膜实现的,特别是当,微氧可以在微尺度级同时提供液相和气相的调制。这项技术使动态多参数实验的关键学习islet病理生理学。

Related Videos

Read Article