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Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis
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Chapters
- 00:05Title
- 02:23Pre-immunohistochemistry
- 03:44Heat-induced Antigen Retrieval
- 04:52Primary and Secondary Antibody Staining
- 06:50Results: Development of Neural Stem Cell During Adult Hippocampal Neurogenesis
- 08:27Conclusion
During adult hippocampal neurogenesis, a distinct set of genetic markers are expressed when a quiescent neural stem cell sequentially progresses and develops into a functionally integrated neuron in the circuit. Using heat-induced antigen retrieval, progenitor cell types that are otherwise difficult to detect are identified with improved effectiveness.
