Journal
/
Biology
/
High Throughput Microinjections af søpindsvin Zygoter
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
High Throughput Microinjections of Sea Urchin Zygotes

High Throughput Microinjections af søpindsvin Zygoter

DOI:
10.3791/50841-v

•

12:40 min

•

January 21, 2014

•

,
1Department of Biological Sciences,University of Delaware

Chapters

  • 00:05Title
  • 01:26Experimental Preparations
  • 04:00Collecting Sea Urchin Gametes
  • 06:12Rowing the Eggs
  • 07:40Microinjection of the Sea Urchin Zygotes
  • 11:13Injection of Reporter Constructs Does Not Affect Development
  • 12:05Conclusion

Summary

Automatic Translation

Automatic Translation

Mikroinjektion er en almindelig teknik, der anvendes til at levere DNA-konstruktioner, mRNA'er, morpholino antisense-oligonucleotider eller andre behandlinger i æg, embryoner, og celler fra forskellige arter.

Tags

MicroinjectionSea Urchin ZygotesHigh-throughputDNA ConstructsMRNAsRecombinant ProteinsGain Of FunctionLoss Of FunctionFluorescent DyeColorimetric DyeCell DeliveryEmbryo DeliveryBiological Processes

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury

High-throughput Yeast Plasmid Overexpression Screen

High-throughput Saccharification Assay for Lignocellulosic Materials

High-throughput Oprensning af Affinity-mærkede rekombinante proteiner

Flowcytometri-baseret oprensning af<em> S. cerevisiae</em> Zygoter

High-throughput Titrering af luciferase-udtrykkende rekombinante vira

Metode til Mikroinjektion af<em> Patiria minata</em> Zygoter

Inddrivelse af voksne zebrafisk Hearts for high-throughput applikationer

High-throughput screening for kemiske modulatorer af post-transkriptionelt regulerede gener

High throughput<em> Danio rerio</em> Energi Udgifter Assay

Read Article