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High Throughput microinjeções de ouriço do mar zigotos
JoVE Journal
Biology
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JoVE Journal Biology
High Throughput Microinjections of Sea Urchin Zygotes
DOI:

12:40 min

January 21, 2014

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Chapters

  • 00:05Title
  • 01:26Experimental Preparations
  • 04:00Collecting Sea Urchin Gametes
  • 06:12Rowing the Eggs
  • 07:40Microinjection of the Sea Urchin Zygotes
  • 11:13Injection of Reporter Constructs Does Not Affect Development
  • 12:05Conclusion

Summary

Automatic Translation

A microinjecção é uma técnica comum usada para fornecer estruturas de ADN, ARNm, oligonucleidos morfolino anti-sentido ou outros tratamentos em ovos, embriões, e as células de várias espécies.

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