Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Coculture System with an Organotypic Brain Slice…
JoVE Journal
Medicine

This content is Open Access.

JoVE Journal Medicine
Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells
 

Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells

Article DOI: 10.3791/50881-v • 07:48 min • October 9th, 2013
October 9th, 2013 •
1, 1, 2, 1, *3, *1
1Department of Hematology and Oncology, University of Göttingen, 2Institute of Neuropathology, University of Göttingen, 3Institute of Anatomy and Cellbiology, University of Halle
* These authors contributed equally

Chapters

Summary
Automatic Translation

The organotypic brain slice coculture with carcinoma cells enables visualizing morphological changes by fluorescence as well as bright field (video) microscopy during the process of carcinoma cell invasion of brain tissue. This model system also allows for cell exchange and replenishment approaches and offers a wide variety of manipulations and analyses.

Tags

Keywords: Coculture Organotypic Brain Slice 3D Spheroid Carcinoma Cells Cerebral Metastasis Tumor Microenvironment Tumor-associated Macrophages (TAM) Astrocytes In Vitro Model Brain Colonization Glial Reactions Molecular Analysis
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter