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Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice
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Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice

Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice

DOI:
10.3791/2716-v

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13:01 min

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August 12, 2011

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,
1Department of Pathology,University of Kansas Medical Center

Chapters

  • 00:05Title
  • 01:33Isolation and Extraction of Donor Collagen from C57BL/6J Mice
  • 04:09Isolation and Culture of Donor Mammary Carcinoma Cells and Fibroblasts from Normal and PyVmT C57BL/6J Mice
  • 06:33Preparation of Collagen Embedded Cells for Grafting
  • 08:32Orthotopic Transplantation of Collagen Embedded Cells in C57BL/6J Mice
  • 10:40Representative Results

Summary

Automatic Translation

Automatic Translation

In this report, we demonstrate a system to isolate and culture donor cells from the mouse mammary gland, and orthotopically transplant these cells in recipient mice to analyze stromal: epithelial interactions during mammary tumor development.

Tags

Mammary TransplantationStromal CellsCarcinoma CellsC57BL/6J MiceTumor ProgressionMouse ModelsEpithelial CellsImmunocompromised MiceExtracellular MatricesMatrigelRat Tail CollagenFunctional T CellsDrug DevelopmentEfficacyOrthotopic TransplantationCarcinoma Associated FibroblastsPyVmT Mammary Carcinoma CellsCollagenInguinal Mammary Glands

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