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Borstklieren transplantatie van stromale cellen en carcinoom cellen in C57BL/6J Muizen
JoVE Journal
Medicine
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JoVE Journal Medicine
Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice
DOI:

13:01 min

August 12, 2011

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Chapters

  • 00:05Title
  • 01:33Isolation and Extraction of Donor Collagen from C57BL/6J Mice
  • 04:09Isolation and Culture of Donor Mammary Carcinoma Cells and Fibroblasts from Normal and PyVmT C57BL/6J Mice
  • 06:33Preparation of Collagen Embedded Cells for Grafting
  • 08:32Orthotopic Transplantation of Collagen Embedded Cells in C57BL/6J Mice
  • 10:40Representative Results

Summary

Automatic Translation

In dit rapport tonen we een systeem te isoleren en cultuur donor cellen van de muis borstklier, en orthotopically deze cellen transplantatie in ontvangende muizen om de stromale analyseren: epitheel interacties tijdens mammatumor ontwikkeling.

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