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C57BL/6J小鼠乳腺间质细胞和癌细胞的移植
JoVE Journal
Medicine
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JoVE Journal Medicine
Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice
DOI:

13:01 min

August 12, 2011

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Chapters

  • 00:05Title
  • 01:33Isolation and Extraction of Donor Collagen from C57BL/6J Mice
  • 04:09Isolation and Culture of Donor Mammary Carcinoma Cells and Fibroblasts from Normal and PyVmT C57BL/6J Mice
  • 06:33Preparation of Collagen Embedded Cells for Grafting
  • 08:32Orthotopic Transplantation of Collagen Embedded Cells in C57BL/6J Mice
  • 10:40Representative Results

Summary

Automatic Translation

在这份报告中,我们展示了一个系统,以隔离和文化的供体细胞,从小鼠乳腺和原位移植受体小鼠,这些细胞分析基质上皮细胞的相互作用:在乳腺肿瘤的发展。

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