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Chapters
- 00:05Title
- 01:33Isolation and Extraction of Donor Collagen from C57BL/6J Mice
- 04:09Isolation and Culture of Donor Mammary Carcinoma Cells and Fibroblasts from Normal and PyVmT C57BL/6J Mice
- 06:33Preparation of Collagen Embedded Cells for Grafting
- 08:32Orthotopic Transplantation of Collagen Embedded Cells in C57BL/6J Mice
- 10:40Representative Results
In questo rapporto, si dimostra un sistema per isolare la cultura e le cellule del donatore dalla ghiandola mammaria del mouse, e ortotopicamente trapianto di queste cellule in topi riceventi per analizzare stromali: interazioni epiteliali durante lo sviluppo del tumore mammario.
Tags
Mammary TransplantationStromal CellsCarcinoma CellsC57BL/6J MiceTumor ProgressionMouse ModelsEpithelial CellsImmunocompromised MiceExtracellular MatricesMatrigelRat Tail CollagenFunctional T CellsDrug DevelopmentEfficacyOrthotopic TransplantationCarcinoma Associated FibroblastsPyVmT Mammary Carcinoma CellsCollagenInguinal Mammary Glands
