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Journal / Biology / Detection of Live Escherichia coli O157:H7 Cells…
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Detection of Live Escherichia coli O157
 

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR

Article DOI: 10.3791/50967-v • 08:16 min • February 1st, 2014
February 1st, 2014 •
1, 1, 1
1Center for Food Safety and Applied Nutrition, Division of Molecular Biology, Food and Drug Administration

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Summary
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A qPCR assay was developed for detection of Escherichia coli O157:H7 targeting a unique genetic marker, Z3276. The qPCR was combined with propidium monoazide (PMA) treatment for live cell detection. This protocol has been modified and adapted to a 96-well plate format for easy and consistent handling of numerous samples

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Live E. Coli O157:H7 Detection ORF Z3276 QPCR Assay PMA-qPCR Live Cell Detection 96-well Plate Format Food Safety Environmental Monitoring
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