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Biology

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Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli (p)ppGpp Followed by Thin Layer Chromatography
 

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli (p)ppGpp Followed by Thin Layer Chromatography

Article DOI: 10.3791/59595-v 06:30 min June 4th, 2019
June 4th, 2019

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Summary

The growth of radiolabeled bacterial cultures in microtiter dishes facilitates high throughput sampling that allows multiple technical and biological replicate assays of nucleotide pool abundance, including that of (p)ppGpp. The effects of growth transitions provoked by sources of physiological stress as well as recovery from stress can be monitored.

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Keywords: Microtiter Dish Radiolabeling Escherichia Coli (p)ppGpp Thin Layer Chromatography Global Regulator Bacterial Cultures Stress MOPS Media P32 Orthophosphate Formic Acid PEI Cellulose Thin Layer Chromatograms
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