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视网膜血流小鼠活体视频显微镜测量
JoVE Journal
Medicine
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JoVE Journal Medicine
Intravital Video Microscopy Measurements of Retinal Blood Flow in Mice
DOI:

10:16 min

December 26, 2013

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Chapters

  • 00:05Title
  • 01:25Preparation of Perfusion Solutions
  • 02:16Animal Anesthesia and Vascular Cannulation
  • 03:45Preparation for Intravital Microscopy
  • 04:41Infusion of Fluorescent Microspheres to Measure Velocities
  • 05:15Infusion of a Fluorescent Plasma Marker to Measure Diameters
  • 06:11Video Analysis
  • 07:56Results: Intravital Microscopy Measurements of Retinal Blood Flow
  • 09:38Conclusion

Summary

Automatic Translation

Intravital microscopy can be used in animals to visualize and measure retinal vascular diameters, bloodstream velocities, and total retinal blood flow.

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