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Chapters
- 00:05Title
- 01:16Cocultivation of Bacteria, Sample Collection, and Sample Storage
- 03:00Sample Treatment, Plating, and Incubation for Quantitative Analyses
- 03:32Counting the Survivors by Stereo Fluorescence Microscopy for Quantitative Analysis
- 04:46Results: Intraspecies Competition
- 06:25Conclusion
Bacteria may accumulate either detrimental or beneficial mutations during their lifetime. In a population of cells individuals that have accumulated beneficial mutations may rapidly outcompete their fellows. Here we present a simple procedure to visualize intraspecies competition in a bacterial cell population over time using fluorescently labeled individuals.
