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通过共生荧光标记菌株监测细菌细胞群中的物种内部竞争
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JoVE Journal Biology
Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains
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通过共生荧光标记菌株监测细菌细胞群中的物种内部竞争

DOI:
10.3791/51196-v

•

06:45 min

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January 18, 2014

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, , ,
1Department of General Microbiology,Georg-August University

Chapters

  • 00:05Title
  • 01:16Cocultivation of Bacteria, Sample Collection, and Sample Storage
  • 03:00Sample Treatment, Plating, and Incubation for Quantitative Analyses
  • 03:32Counting the Survivors by Stereo Fluorescence Microscopy for Quantitative Analysis
  • 04:46Results: Intraspecies Competition
  • 06:25Conclusion

Summary

Automatic Translation

Automatic Translation

细菌在有生之年可能会积累有害或有益的突变。在积累有益突变的细胞群中，个体可能迅速比其研究员竞争。在这里，我们介绍了一个简单的程序，以可视化物种内部竞争的细菌细胞种群随着时间的推移使用荧光标记的个人。

Tags

Intraspecies CompetitionBacterial Cell PopulationFluorescently Labeled StrainsBacillus SubtilisClonal ExpansionBeneficial MutationsDetrimental MutationsSelectionGenotype ShiftPopulation DynamicsCocultivationMicrobial Competition

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