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新生儿软脑膜表面电穿孔
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Neonatal Pial Surface Electroporation
DOI:

06:22 min

May 07, 2014

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Chapters

  • 00:05Title
  • 01:21Preparation
  • 02:36Pial Surface Plasmid Injection
  • 03:38Electroporation
  • 05:27Results: Pial Surface Expression
  • 05:58Conclusion

Summary

Automatic Translation

软脑膜表面是受到越来越多的关注,在中枢神经系统的唯一祖区。在此,我们详细为这个祖区使用改进的电穿孔法快速基因操作的方法。此过程可用于细胞和分子细胞谱系及参与细胞分化信号通路的调查,并阐明子细胞的命运和属性。

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