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隔离成年小鼠心肌细胞的细胞信号传导和文化及<em>在体外</em>心肌肥厚

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Biology

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JoVE Journal Biology

Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy

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隔离成年小鼠心肌细胞的细胞信号传导和文化及<em>在体外</em>心肌肥厚

DOI:

10.3791/51357-v

•

06:51 min

•

May 21, 2014

•

Daxiang Li, Jian Wu, Yan Bai, Xiaochen Zhao, Lijun Liu

¹Department of Biochemistry and Cancer Biology,University of Toledo College of Medicine and Life Sciences, ²Department of Physiology and Pharmacology,University of Toledo College of Medicine and Life Sciences

Chapters

00:05Title
01:12Mouse Heart Removal and Cannulation
02:54Heart Perfusion and Digestion
03:25Cells Dissociation and Calcium Reintroduction
05:10Cell Culture
06:00Results: Successful Isolation Quantification
06:29Conclusion

Summary

Automatic Translation

我们描述了成年小鼠心肌细胞分离的可靠方法。该协议得到了一致的结果为从各种转基因小鼠的功能成年心肌文化。

Tags

Adult Mouse Cardiomyocytes Cell Signaling Cardiac Hypertrophy Genetically Modified Mice Cardiomyocyte Isolation Langendorff Perfusion Type II Collagenase Cardiovascular Research Drug Development

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