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隔离成年小鼠心肌细胞的细胞信号传导和文化及<em>在体外</em>心肌肥厚
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Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and
in vitro
Cardiac Hypertrophy
Please note that all translations are automatically generated.
Click here for the English version.
隔离成年小鼠心肌细胞的细胞信号传导和文化及<em>在体外</em>心肌肥厚
DOI:
10.3791/51357-v
•
06:51 min
•
May 21, 2014
•
Daxiang Li
,
Jian Wu
,
Yan Bai
,
Xiaochen Zhao
,
Lijun Liu
1
Department of Biochemistry and Cancer Biology
,
University of Toledo College of Medicine and Life Sciences
,
2
Department of Physiology and Pharmacology
,
University of Toledo College of Medicine and Life Sciences
Chapters
00:05
Title
01:12
Mouse Heart Removal and Cannulation
02:54
Heart Perfusion and Digestion
03:25
Cells Dissociation and Calcium Reintroduction
05:10
Cell Culture
06:00
Results: Successful Isolation Quantification
06:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们描述了成年小鼠心肌细胞分离的可靠方法。该协议得到了一致的结果为从各种转基因小鼠的功能成年心肌文化。
Tags
Adult Mouse Cardiomyocytes
Cell Signaling
Cardiac Hypertrophy
Genetically Modified Mice
Cardiomyocyte Isolation
Langendorff Perfusion
Type II Collagenase
Cardiovascular Research
Drug Development
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