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在Wholemount大鼠视网膜免疫组化和钙成像方法
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina
Please note that all translations are automatically generated.
Click here for the English version.
在Wholemount大鼠视网膜免疫组化和钙成像方法
DOI:
10.3791/51396-v
•
08:54 min
•
October 13, 2014
•
Allison Sargoy
,
Steven Barnes
2,3
,
Nicholas C. Brecha
2,4
,
Luis Pérez De Sevilla Müller
1
Department of Neurobiology
,
University of California, Los Angeles
,
2
Veterans Administration Greater Los Angeles Healthcare System
,
3
Departments of Physiology & Biophysics and Ophthalmology & Visual Sciences
,
Dalhousie University
,
4
Departments of Neurobiology and Medicine, Jules Stein Eye Institute, CURE-Digestive Diseases Research Center, David Geffen School of Medicine
,
University of California, Los Angeles
Chapters
00:05
Title
01:25
Eyecup Preparation
02:58
Wholemount Retina Preparation and Processing
05:06
Calcium Imaging in Retinal Ganglion Cells
07:19
Results: RGC Immunostaining and Electrophysiology
08:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
免疫组化协议,用于研究在视网膜特定蛋白质的定位。钙成像技术被用来研究视网膜神经节细胞及其轴突钙的动态。
Tags
Immunohistochemistry
Calcium Imaging
Wholemount Retina
Retinal Ganglion Cells
Voltage-gated Calcium Channels
Displaced Amacrine Cells
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