Journal
/
Neuroscience
/
Immunhistokemiske og Calcium Imaging Methods in whole- mount rotteretina
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina

Immunhistokemiske og Calcium Imaging Methods in whole- mount rotteretina

DOI:
10.3791/51396-v

•

08:54 min

•

October 13, 2014

•

, , ,
1Department of Neurobiology,University of California, Los Angeles, 2Veterans Administration Greater Los Angeles Healthcare System, 3Departments of Physiology & Biophysics and Ophthalmology & Visual Sciences,Dalhousie University, 4Departments of Neurobiology and Medicine, Jules Stein Eye Institute, CURE-Digestive Diseases Research Center, David Geffen School of Medicine,University of California, Los Angeles

Chapters

  • 00:05Title
  • 01:25Eyecup Preparation
  • 02:58Wholemount Retina Preparation and Processing
  • 05:06Calcium Imaging in Retinal Ganglion Cells
  • 07:19Results: RGC Immunostaining and Electrophysiology
  • 08:29Conclusion

Summary

Automatic Translation

Automatic Translation

Immunhistokemi protokoller anvendt til at undersøge lokalisering af et specifikt protein i nethinden. Calcium billeddiagnostiske teknikker er ansat til at studere calcium dynamik i retina-ganglieceller og deres axoner.

Tags

Keywords: ImmunohistochemistryCalcium ImagingWholemount RetinaRetinal Ganglion CellsVoltage-gated Calcium ChannelsDisplaced Amacrine Cells

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51396?article_type=v

Funktionel Calcium Imaging i udviklingslandene Kortikal Networks
check_url/51396?article_type=v

Wholemount Immunhistokemi for at afsløre Complex Brain Topografi
check_url/51396?article_type=v

In vivo Neuronal Calcium Imaging i C. elegans
check_url/51396?article_type=v

Immunohistokemisk analyse i Rat centralnervesystemet og det perifere lymfeknude Vævssnit
check_url/51396?article_type=v

Ikke-invasiv Parenkymalt, vaskulære og metaboliske højfrekvent ultralyd og Photoacoustic Rat Deep Brain Imaging
check_url/51396?article_type=v

Transpupillær to-foton In vivo Billeddannelse af musens nethinde
check_url/51396?article_type=v

In vivo Calcium Imaging i Mus Ringere Olive
check_url/51396?article_type=v

Hjerne pericyt calcium og hæmodynamisk billeddannelse i transgene mus In Vivo
check_url/51396?article_type=v

Subcellulær billeddannelse af neuronal calciumhåndtering in vivo
check_url/51396?article_type=v

Calciumbilleddannelse i mus overlegen colliculus

Read Article