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重组β-catenin的降解<em>爪蟾</em>卵提取物
JoVE Journal
Biology
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JoVE Journal Biology
Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
DOI:

09:41 min

June 17, 2014

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Chapters

  • 00:05Title
  • 01:41Depletion from Xenopus Extract
  • 02:57Optimizing Xenopus Extract for β-catenin Degradation
  • 04:10Radiolabeled β-catenin Degradation Assay in Xenopus Egg Extract
  • 05:47β-catenin-luciferase Degradation Assay in Xenopus Egg Extract
  • 07:24Results: Analysis of β-catenin Degradation
  • 09:03Conclusion

Summary

Automatic Translation

的方法被描述为使用放射性标记和荧光素酶融合蛋白在非洲蟾蜍卵提取物和其适应高通量筛选用于蛋白质降解的小分子调节剂进行分析的蛋白质降解。

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