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Rekonstituering af β-catenin nedbrydning i<em> Xenopus</em> Æggeekstrakt
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Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Rekonstituering af β-catenin nedbrydning i<em> Xenopus</em> Æggeekstrakt

DOI:
10.3791/51425-v

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09:41 min

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June 17, 2014

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, , ,
1Department of Cell and Developmental Biology and Program in Developmental Biology,Vanderbilt University Medical Center, 2Division of Gastroenterology, Hepatology & Nutrition and Division of Developmental Biology,Cincinnati Children's Hospital Medical Center, 3Vanderbilt Ingram Cancer Center,Vanderbilt University School of Medicine

Chapters

  • 00:05Title
  • 01:41Depletion from Xenopus Extract
  • 02:57Optimizing Xenopus Extract for β-catenin Degradation
  • 04:10Radiolabeled β-catenin Degradation Assay in Xenopus Egg Extract
  • 05:47β-catenin-luciferase Degradation Assay in Xenopus Egg Extract
  • 07:24Results: Analysis of β-catenin Degradation
  • 09:03Conclusion

Summary

Automatic Translation

Automatic Translation

Der beskrives en fremgangsmåde til analyse af protein-nedbrydning under anvendelse af radioaktivt mærket og luciferase-fusionsproteiner i Xenopus æggeekstrakt og dens tilpasning til high-throughput screening for små molekyler modulatorer af proteinnedbrydning.

Tags

Keywords: Xenopus Egg Extractβ-catenin DegradationProtein TurnoverCell CycleSignal TransductionWnt Pathway[35S]-radiolabeled ProteinsFirefly Luciferase-tagged Fusion ProteinsHigh-throughput Screening

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