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ScanLag: High-throughput Kwantificering van Colony Groei en vertraging
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
ScanLag: High-throughput Quantification of Colony Growth and Lag Time
DOI:

07:47 min

July 15, 2014

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Chapters

  • 00:05Title
  • 01:18Build the Setup
  • 02:24Performing an Experiment Using the Scanning Manager
  • 03:57Analysis of the Images
  • 05:52Results: Quantitative Analysis of Colony Growth and Lag Time with ScanLag
  • 07:18Conclusion

Summary

Automatic Translation

ScanLag is a high-throughput method for measuring the delay in growth, namely lag time, as well as the growth rate of colonies for thousands of cells in parallel. Screening using ScanLag enables the discrimination between long lag-time and slow growth at the level of a single variant.

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