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Immunology and Infection

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JoVE Journal Immunology and Infection
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
 

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Article DOI: 10.3791/51492-v • 12:55 min • January 17th, 2015
January 17th, 2015 •
1,2, 1,2, 1,2, 1,2, 1,3, 1,3, 1,4, 1,4, 1,3, 1,4, 1,4, 1,2
1The Recombinant Antibody Network, 2The Banting and Best Department of Medical Research, University of Toronto, 3Antibiome Center, University of California, San Francisco at Mission Bay, 4Department of Biochemistry and Molecular Biology, The University of Chicago

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Summary
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A method is described with visual accompaniment for conducting scalable, high throughput selections from phage-displayed combinatorial synthetic antibody libraries against hundreds of antigens simultaneously. Using this parallel approach, we have isolated antibody fragments that exhibit high affinity and specificity for diverse antigens that are functional in standard immunoassays.

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Keywords: Phage Display Synthetic Antibody Libraries High-throughput Selection Fab Fragments Antigen Characterization Antibody Discovery Immunoassays
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