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Immunology and Infection

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JoVE Journal Immunology and Infection
Targeted Antibody Blocking by a Dual-Functional Conjugate of Antigenic Peptide and Fc-III Mimetics (DCAF)
 

Targeted Antibody Blocking by a Dual-Functional Conjugate of Antigenic Peptide and Fc-III Mimetics (DCAF)

Article DOI: 10.3791/60063-v • 09:39 min • September 17th, 2019
September 17th, 2019 •
*1,2, *3, 2, 2, 2, 3, 1,2
1Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, 2Mass Spectrometry Core Facility, School of Life Sciences, Westlake University, 3MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University
* These authors contributed equally

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Summary
Automatic Translation

Development of a dual-functional conjugate of antigenic peptide and Fc-III mimetics (DCAF) is novel for the elimination of harmful antibodies. Here, we describe a detailed protocol for the synthesis of DCAF1 molecule, which can selectively block 4G2 antibody to eliminate antibody dependent enhancement effect during Dengue virus infection.

Tags

Keywords: DCAF Antibody Blocking Targeted Antibody Neutralization Peptide Synthesis Fc-III Peptide SUMO Tagged Protein SUMO Protease Nickel NTA Bead
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