A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences,Westlake University, 2Mass Spectrometry Core Facility, School of Life Sciences,Westlake University, 3MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Sciences,Tsinghua University
Chapters
- 00:05Title
- 00:57Chemical Synthesis of the Hydrazine Derivative of a Fc-III Peptide
- 02:31Cleavage of the SUMO Tag
- 04:09Assembling of DCAF1 by Native Chemical Ligation
- 07:06Mass Spectrometry and ELISA Assay
- 08:19Results: Native Chemical Ligation and ELISA Assay
- 09:03Conclusion
