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Målrettet antistof blokering ved et dobbelt funktionelt konjugat af antigene peptid og FC-III-Mimetika (DCAF)
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1Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences,Westlake University, 2Mass Spectrometry Core Facility, School of Life Sciences,Westlake University, 3MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Sciences,Tsinghua University
Chapters
- 00:05Title
- 00:57Chemical Synthesis of the Hydrazine Derivative of a Fc-III Peptide
- 02:31Cleavage of the SUMO Tag
- 04:09Assembling of DCAF1 by Native Chemical Ligation
- 07:06Mass Spectrometry and ELISA Assay
- 08:19Results: Native Chemical Ligation and ELISA Assay
- 09:03Conclusion
Udvikling af en dual-funktionelle konjugat af antigene peptid og FC-III MIME (DCAF) er en roman til eliminering af skadelige antistoffer. Her beskriver vi en detaljeret protokol for syntesen af DCAF1 molekyle, som selektivt kan blokere 4G2 antistof for at eliminere antistof afhængige ekstraudstyr effekt under dengue virus infektion.
