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Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
DOI:

08:35 min

February 21, 2014

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Chapters

  • 00:05Title
  • 01:20Infinity Chamber Fabrication
  • 02:40Turning and Inkjet into a Lipidjet
  • 04:50Assembling and Using the Equipment
  • 07:24Vesicle Formation
  • 08:15Conclusion

Summary

Automatic Translation

Microfluidic jetting against a droplet interface lipid bilayer provides a reliable way to generate vesicles with control over membrane asymmetry, incorporation of transmembrane proteins, and encapsulation of material. This technique can be applied to study a variety of biological systems where compartmentalized biomolecules are desired.

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