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细胞质DNA的检测途径的刺激<em>在体外</em>和<em>体内</em
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JoVE Journal Biology
Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo
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细胞质DNA的检测途径的刺激<em>在体外</em>和<em>体内</em

DOI:
10.3791/51593-v

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11:44 min

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September 18, 2014

•

,
1Department of Pathology,University of Cambridge

Chapters

  • 00:05Title
  • 01:17Concatemerization of Immuno-stimulatory DNA Sequences
  • 02:43DNA Purification and Analysis
  • 05:55Transfection of dsDNA Concatemers In Vitro
  • 06:47Transfection of dsDNA Concatemers In Vivo
  • 08:29Processing of DNA Stimulated Samples and Quantitative PCR Analysis
  • 10:32Results: Concatermerized DNA Stimulate an Innate Immune Response In Vitro and In Vivo
  • 11:14Conclusion

Summary

Automatic Translation

Automatic Translation

该协议的目的是要使用的最佳方法用于刺激细胞质DNA传感途径在细胞和体内 。这是通过平末端连接时改善长时间，双链DNA的生成来实现的。细胞或小鼠用脂质转染试剂转染。

Tags

DNA Sensing PathwaysCytoplasmic DNADouble-stranded DNAOligonucleotidesConcatemerizationPolyethylene GlycolInnate Immune ResponseIn VitroIn VivoTransfectionIntradermal Injection

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