/

/

新生儿肝外胆管的隔离

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Isolation of Neonatal Extrahepatic Cholangiocytes

Please note that all translations are automatically generated. Click here for the English version.

新生儿肝外胆管的隔离

DOI:

10.3791/51621-v

•

07:54 min

•

June 05, 2014

•

Sara Karjoo, Rebecca G. Wells

¹Division of Gastroenterology, Hepatology, and Nutrition,The Children’s Hospital of Philadelphia, ²Department of Medicine,The Perelman School of Medicine at the University of Pennsylvania

Chapters

00:05Title
01:12Bile Duct Isolation
02:59Embedding Tissues into Thick Collagen Gels
04:19Isolating Primary Cholangiocytes
07:05Characterizing Cultured Cholangiocytes
07:24Conclusion

Summary

Automatic Translation

一种技术，从新生小鼠的肝外胆管胆管隔离描述。管道正在认真解剖，然后细胞被生长在厚厚的胶原凝胶中分离。该方法为研究肝外胆管发育和病理的有用工具。

Tags

Keywords: Neonatal Extrahepatic Cholangiocytes Bile Ducts Intrahepatic Extrahepatic Cell Isolation Collagen Cell Culture Trypsinization Cell Purification Biliary Atresia

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

瓣膜内皮细胞的分离

从新生小鼠的肺血管内皮细胞的分离和培养

分离果蝇睾丸

分离新生小鼠心肌文化和

肺动脉平滑肌细胞从新生小鼠隔离

原代大鼠乳鼠心肌细胞的活细胞成像继腺病毒和慢病毒转导利用共聚焦旋转盘显微镜

鼠阀门内皮细胞的分离

在新生小鼠静脉注射

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

小鼠巨核细胞祖细胞的分离

Read Article