JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Bioengineering
/
谷氨酰胺通量成像使用基因编码的传感器
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Bioengineering
Glutamine Flux Imaging Using Genetically Encoded Sensors
Please note that all translations are automatically generated.
Click here for the English version.
谷氨酰胺通量成像使用基因编码的传感器
DOI:
10.3791/51657-v
•
10:23 min
•
July 31, 2014
•
Julien Besnard
,
Sakiko Okumoto
1
Virginia Tech
Chapters
00:05
Title
01:22
Sample Preparation
03:32
Perfusion Experiment
08:01
Results: Glutamine Sensors Detect Transporter Activities in a Single Cell
09:53
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本文将展示如何利用FRET监测谷氨酰胺动态活细胞。遗传编码的传感器允许生物分子的亚细胞分辨率的实时监控。实验设计,实验设置的技术细节,并考虑后期的实验分析将用于基因编码的谷氨酰胺的传感器进行讨论。
Tags
Keywords: Genetically Encoded Sensors
Biological Molecules
Subcellular Resolution
Cellular Transport Processes
Transporter Dynamics
Glutamine Sensor
Experimental Design
Technical Details
Post-experimental Analyses
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/51657?article_type=v
使用电化学传感器的细菌检测和鉴定
check_url/51657?article_type=v
微流体为基础的电化学生物芯片的无标记的DNA杂交分析
check_url/51657?article_type=v
飞行昆虫检测与分类用便宜的传感器
check_url/51657?article_type=v
基因转染向着文化微模式球体板细胞基因修饰的细胞移植
check_url/51657?article_type=v
使用多通道双电场微流控芯片肺癌细胞Electrotaxis研究
check_url/51657?article_type=v
从混合三维培养使用近红外Photoimmunotherapy技术选择性细胞消除
check_url/51657?article_type=v
细胞内胆汁酸动态实时监控使用遗传编码的FRET为基础的胆汁酸传感器
check_url/51657?article_type=v
多显微镜下的血管生成术在基因修饰的 3 d-PLGA/nHAp 支架上进行颅骨临界骨缺损修复
check_url/51657?article_type=v
CRISPR/Cas9 复合原注射液生产基因工程金黄叙利亚仓鼠
check_url/51657?article_type=v
应用交互式、患者特异性模型对神经纤维束进行深部脑刺激治疗的靶向性
Read Article