Journal
/
/
谷氨酰胺通量成像使用基因编码的传感器
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Glutamine Flux Imaging Using Genetically Encoded Sensors
DOI:

10:23 min

July 31, 2014

,

Chapters

  • 00:05Title
  • 01:22Sample Preparation
  • 03:32Perfusion Experiment
  • 08:01Results: Glutamine Sensors Detect Transporter Activities in a Single Cell
  • 09:53Conclusion

Summary

Automatic Translation

本文将展示如何利用FRET监测谷氨酰胺动态活细胞。遗传编码的传感器允许生物分子的亚细胞分辨率的实时监控。实验设计,实验设置的技术细节,并考虑后期的实验分析将用于基因编码的谷氨酰胺的传感器进行讨论。

Related Videos

Read Article