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多步制备技术,以恢复多个代谢化合物的种类进行深入和信息化代谢组学分析
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
DOI:

11:25 min

July 11, 2014

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Chapters

  • 00:05Title
  • 01:22Protein Precipitation
  • 04:21Liquid-liquid Extraction
  • 06:55Solid-phase Extraction
  • 09:12Results: Metabolites are Effectively Extracted from Biological Fluids by this Protocol
  • 10:52Conclusion

Summary

Automatic Translation

结果在代谢组学实验的可靠性取决于样品制备的有效性和可重复性。描述的是一个严格和深入的方法,使从生物体液提取的代谢物与随后分析多达数千种化合物中,或感兴趣的只是该化合物类的选项。

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