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Chapters
- 00:05Title
- 01:29Determination of an Approximate Value for the Dissociation Constant
- 04:32Experimental Set-up for Determining the Dissociation Constant
- 08:55Data Analysis to Determine the Dissociation Constant Under Thermal Denaturation
- 10:15Results: Differential Scanning Fluorimetry Measures Protein-ligand Interactions
- 12:40Conclusion
Differential scanning fluorimetry is a widely used method for screening libraries of small molecules for interactions with proteins. Here, we present a straightforward method to extend these analyses to provide an estimate of the dissociation constant between a small molecule and its protein partner.
