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订货单细胞和单胚胎的三维约束:高内涵筛选新设备
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening
DOI:

14:22 min

September 18, 2016

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Chapters

  • 00:05Title
  • 02:29Microfabrication of ‘Eggcups’
  • 06:29Introducing Cells into the ‘Eggcups’
  • 09:31Observation of Fixed Cellular Organelles in ‘Eggcups’
  • 11:22Results: ‘Eggcups’ Enable Visualization of Cellular Organelles and Study of Dynamic Cell Processes
  • 13:27Conclusion

Summary

Automatic Translation

我们报告的设备和新的方法来研究细胞和胚胎。单细胞精确下令微阵列。他们的3D坐月子是朝着在生理条件下遇到的3D环境的一个步骤,并允许细胞器的方向。通过控制细胞的形状,此设置最大限度地减少可变性中的标准试验的报道。

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