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Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models
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Chapters
- 00:05Title
- 02:05Pre-surgical Preparation
- 02:55In Utero Electroporation
- 06:49Harvesting Brains
- 08:35Co-staining with anti-GFP
- 09:29Results: Confirming Cre-mediated Gene Excision in Electroporated Neurons
- 11:14Conclusion
Neuron migration is regulated by numerous cell autonomous and non-cell autonomous factors. This protocol shows how in utero electroporation can be used to determine whether a phenotype in a transgenic mouse is due to disruption of a cell intrinsic mechanism or impairment of interaction between the neuron and its environment.
