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小鼠骨髓破骨细胞的推导

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Biology

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JoVE Journal Biology

Osteoclast Derivation from Mouse Bone Marrow

Please note that all translations are automatically generated. Click here for the English version.

小鼠骨髓破骨细胞的推导

DOI:

10.3791/52056-v

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06:17 min

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November 06, 2014

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Ruth Tevlin*¹, Adrian McArdle*^1,2, Charles K.F. Chan, John Pluvinage, Graham G. Walmsley², Taylor Wearda², Owen Marecic², Michael S. Hu, Kevin J. Paik, Kshemendra Senarath-Yapa, David A. Atashroo, Elizabeth R. Zielins, Derrick C. Wan, Irving L. Weissman², Michael T. Longaker²

¹Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery,Stanford University School of Medicine, ²Institute for Stem Cell Biology and Regenerative Medicine,Stanford University

Chapters

00:05Title
01:28Media Preparation and Bone Marrow Isolation
03:05Gradient Separation of Bone Marrow Cells and Cell Culturing
04:57Results: Culturing Osteoclasts from Mouse Bone Marrow
05:56Conclusion

Summary

Automatic Translation

破骨细胞是体内的主要骨吸收细胞。一个分离的破骨细胞中大量的能力已经导致破骨细胞生物学的理解显著进步。在这个协议中，我们描述了一种用于分离，培养和定量破骨细胞的体外活性。

Tags

Keywords: Osteoclast Bone Marrow Monocyte/macrophage Lineage Bone Resorption Bone Remodeling Osteoblasts Skeletal Remodeling Bone Matrix Bone Formation Bone Disease Osteoporosis In Vitro Osteoclast Isolation Bone Remodeling Cycle Therapeutic Strategies

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