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Osteoclast Derivation fra Mouse Bone Marrow
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JoVE Journal Biology
Osteoclast Derivation from Mouse Bone Marrow

Osteoclast Derivation fra Mouse Bone Marrow

DOI:
10.3791/52056-v

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06:17 min

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November 06, 2014

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, , , , , , , , , , , , , ,
1Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery,Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine,Stanford University

Chapters

  • 00:05Title
  • 01:28Media Preparation and Bone Marrow Isolation
  • 03:05Gradient Separation of Bone Marrow Cells and Cell Culturing
  • 04:57Results: Culturing Osteoclasts from Mouse Bone Marrow
  • 05:56Conclusion

Summary

Automatic Translation

Automatic Translation

Osteoklaster er den primære knogleresorberende celle i kroppen. En evne til at isolere osteoklaster i stort tal har resulteret i betydelige fremskridt i forståelsen af ​​osteoclast biologi. I denne protokol, beskriver vi en metode til isolering, dyrkning og kvantificere osteoklastaktivitet in vitro.

Tags

Keywords: OsteoclastBone MarrowMonocyte/macrophage LineageBone ResorptionBone RemodelingOsteoblastsSkeletal RemodelingBone MatrixBone FormationBone DiseaseOsteoporosisIn Vitro Osteoclast IsolationBone Remodeling CycleTherapeutic Strategies

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