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Högupplöst elektronmikroskopi av<em> Helicobacter pylori</em> Cag Typ IV sekretionssystemet Pili Producerad i varierande förhållanden of Iron Tillgänglighet
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
High Resolution Electron Microscopy of the Helicobacter pylori Cag Type IV Secretion System Pili Produced in Varying Conditions of Iron Availability
DOI:

09:05 min

November 21, 2014

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Chapters

  • 00:05Title
  • 01:36H. pylori, Iron Availability and AGS Co-culture
  • 03:24SEM Sample Preparation
  • 05:01Imaging Parameters for High Resolution SEM Analyses
  • 05:46Quantifying H. pylori Cag-T4SS Pili
  • 07:07Results: Effect of Iron on H. pylori Cag-T4SS Pilus Biogenesis
  • 08:31Conclusion

Summary

Automatic Translation

Here we describe a method to visualize the oncogenic bacterial organelle known as the Cag Type IV Secretion System (Cag-T4SS). We find that the Cag-T4SS is differentially produced on the surface of H. pylori in response to varying conditions of iron availability.

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