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Método quirúrgico para la entrega de genes mediada por virus de la oreja del ratón Interior a través de la ventana de membrana Ronda
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Surgical Method for Virally Mediated Gene Delivery to the Mouse Inner Ear through the Round Window Membrane
DOI:

07:32 min

March 16, 2015

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Chapters

  • 00:05Title
  • 01:34Surgical Procedure
  • 05:15Results: Transgene Expression Using Immunofluorescence and Hearing Evaluation Using ABR
  • 06:50Conclusion

Summary

Automatic Translation

The described post-auricular surgical approach allows rapid and direct delivery into the mouse cochlear scala tympani while minimizing blood loss and animal mortality. This method can be used for cochlear therapy using molecular, pharmacologic and viral delivery to postnatal mice through the round window membrane.

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