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Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method
 

Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method

Article DOI: 10.3791/52211-v • 09:54 min • December 16th, 2014
December 16th, 2014 •
1, 1
1Division of Neuropathology, Department of Pathology, Pathobiology Graduate Program, Johns Hopkins School of Medicine

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Summary
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This video describes dissection, tissue processing, sectioning, and fluorescence-based TUNEL labeling of mouse skeletal muscle. It also describes a method for semi-automated analysis of TUNEL labeling.

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Keywords: TUNEL DNA Damage Mouse Skeletal Muscle Tissue Processing Fluorescence Apoptosis Quantitation
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