A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Division of Pulmonary and Critical Care Medicine,Brigham and Women’s Hospital – Harvard Medical School, 2Department of Respiratory Medicine,University of Cambridge – Addenbrooke’s Hospital, 3Lung Transplant Program,Brigham and Women’s Hospital – Harvard Medical School, 4COPD and IPF Programs,Lovelace Respiratory Research Institute
Chapters
- 00:05Title
- 01:29Pulmonary Function Test and Lung Inflation
- 03:57Lung Inflation
- 05:04Morphometry
- 07:19Analyze the Reports Using Excel Report Macros
- 08:18Results: Automated Pulmonary Emphysema Measurements in Cigarette Smoke-exposed Mice
- 09:55Conclusion
The goal of this protocol is to provide automated methods to quantify chronic lung pathologies in a murine model of COPD. The protocol includes exposing mice to cigarette smoke (CS), measuring pulmonary function, inflating the lungs, and using morphometry methods to measure emphysema and small airway remodeling in mice.
