Journal
/
Immunology and Infection
/
Cultivation of Heligmosomoides Polygyrus: An Immunomodulatory Nematode Parasite and its Secreted Products
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Cultivation of Heligmosomoides Polygyrus: An Immunomodulatory Nematode Parasite and its Secreted Products

Cultivation of Heligmosomoides Polygyrus: An Immunomodulatory Nematode Parasite and its Secreted Products

DOI:
10.3791/52412-v

•

12:40 min

•

April 06, 2015

•

, , , , , , ,
1Institute of Immunology and Infection Research,University of Edinburgh, 2Manchester Collaborative Centre for Inflammation Research

Chapters

  • 00:05Title
  • 01:49Infection of Mice by Gavage
  • 02:55Collection of Adult H. polygyrus Worms
  • 06:06Propagation of H. polygyrus Life Cycle
  • 07:22Setting Up Cultures for HES
  • 07:57Preparation of HES
  • 10:24Results: The H. polygyrus Life Cycle Can Be Maintained Long Term and Their Excretory-secretory Products Collected
  • 11:33Conclusion

Summary

Automatic Translation

Automatic Translation

Heligmosomoides polygyrus is a murine nematode with powerful immunomodulatory capabilities that closely resemble those of highly-prevalent human helminth infection. Here we describe a protocol for the long-term maintenance of the H. polygyrus lifecycle.

Tags

Keywords: Heligmosomoides PolygyrusNematospiroides DubiusH. BakeriGastrointestinal HelminthImmunomodulatoryChronic InfectionMiceHelminth-derived Immune RegulationAllergyAutoimmunityL3 LarvaeAdult ParasitesExcretory-secretory ProductsHES

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52412?article_type=v

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow
check_url/52412?article_type=v

Parasite Induced Genetically Driven Autoimmune Chagas Heart Disease in the Chicken Model
check_url/52412?article_type=v

A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line
check_url/52412?article_type=v

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
check_url/52412?article_type=v

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)
check_url/52412?article_type=v

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
check_url/52412?article_type=v

Development of an Economical DNA Delivery System by “Acufection” and its Application to Skin Research
check_url/52412?article_type=v

Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs
check_url/52412?article_type=v

Generation of Monoclonal Antibodies Against Natural Products
check_url/52412?article_type=v

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

Read Article